Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2122Y] to Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558)
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y]
    See all Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) primary antibodies

  • Description

    Rabbit monoclonal [EP2122Y] to Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558)

  • Host species

    Rabbit

  • Specificity

    ab76247 will detect Ezrin, Radixin and Moesin. This antibody detects the phosphorylated target and not the non-phosphorylated epitope.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558). The exact sequence is proprietary. A synthetic peptide corresponding to residues surrounding Thr567 (Ezrin), Thr564 (Radixin) and Thr558 (Moesin) of human Ezrin.

  • Positive control

    • WB: HeLa, HEK-293, NIH/3T3, and C6 starved overnight, then treated with 100nM Calyculin A whole cell lysates. IHC-P: Human breast, mouse testis, and rat liver tissues. Dot Blot: Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) phospho peptide and Non-phospho peptide.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    All lanes : Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247) at 1/1000 dilution

    Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
    Lane 4 : Untreated HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 5 : HEK-293 (Human embryonic kidney epithelial cell) starved overnight, then treated with 100nM Calyculin A for 60 minutes whole cell lysate
    Lane 6 : HEK-293 (Human embryonic kidney epithelial cell) starved overnight, then treated with 100nM Calyculin A for g0 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa
    Observed band size: 75-81 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) with ab76247 at 1/500 (1.64 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Membranous staining on human breast.

    The section was incubated with ab76247 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Counterstained with Hematoxylin.

  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    All lanes : Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247) at 1/1000 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embyonic fibroblast) whole cell lysate
    Lane 2 : Untreated NIH/3T3 (Mouse embyonic fibroblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate
    Lane 3 : Untreated NIH/3T3 (Mouse embyonic fibroblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa
    Observed band size: 75-81 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) with ab76247 at 1/500 (1.64 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Membranous staining on human breast.

    The section was incubated with ab76247 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Counterstained with Hematoxylin.

  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    All lanes : Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247) at 1/1000 dilution

    Lane 1 : Untreated C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 2 : Untreated C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes whole cell lysate
    Lane 3 : Untreated C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa
    Observed band size: 75-81 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) with ab76247 at 1/500 (1.64 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Membranous staining on human breast.

    The section was incubated with ab76247 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Counterstained with Hematoxylin.

  • Dot Blot - Anti-Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) antibody [EP2122Y] (ab76247)
    Dot Blot - Anti-Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) antibody [EP2122Y] (ab76247)

    Dot blot analysis of Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) phospho peptide (Lane 1), Non-phospho peptide (Lane 2), labelling Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) with ab76247 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The sequence of non-phospho peptide: CGRDKYKTLRQIR.
    The sequence of phospho peptide: CGRDKYK-pT-LRQIR.

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