Alsterpaullone, cyclin-dependent kinase (CDK) and GSK-3beta inhibitor (ab141070)
Key features and details
- Potent cyclin-dependent kinase (CDK) and GSK-3β inhibitor
- CAS Number: 237430-03-4
- Purity: > 96%
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Alsterpaullone, cyclin-dependent kinase (CDK) and GSK-3beta inhibitor -
Description
Potent cyclin-dependent kinase (CDK) and GSK-3β inhibitor -
Biological description
Potent CDK inhibitor (IC50 values are 3.5 (CDK1/cyclin B), 20 (CDK2/cyclin A) and 40 nM (CDK5/p35)). Also displays potent and selective inhibition of GSK-3β (IC50 values are 40 and 4 nM respectively). Antiproliferative effects seen in vitro. Cell-permeable. -
Purity
> 96% -
CAS Number
237430-03-4 -
Chemical structure
Images
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Functional Studies - Alsterpaullone, cyclin-dependent kinase (CDK) and GSK-3beta inhibitor (ab141070)ab32505 staining AKT1 in SK-N-SH cells treated with alsterpaullone (ab141070), by ICC/IF. Decrease of AKT1 expression correlates with increased concentration of alsterpaullone, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab141070 (alsterpaullone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32505 (1/200 dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
