Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15581-54] to TOMM20 - Mitochondrial Marker
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker
    See all TOMM20 primary antibodies

  • Description

    Rabbit monoclonal [EPR15581-54] to TOMM20 - Mitochondrial Marker

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, HeLa, SH-SY5Y, PC-12 and NIH 3T3 cell lysates; IHC-P: Human ovarian carcinoma and mouse cardiac muscle tissues; ICC/IF: HeLa cells; Flow Cyt: HeLa cells; IHC-Fr: Mouse cardiac and small intestine tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    All lanes : Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) at 1/10000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Immunohistochemistry (Frozen sections) analysis of rat small intestine tissue sections labeling TOMM20 with Purified ab186735 at 1/50 (1.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

    ab186735 staining TOMM20 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Control: PBS only

  • Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    All lanes : Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) at 1/2000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
    Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling TOMM20 with ab186735 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Flow Cytometry - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

    Flow cytometry analysis of TOMM20 expression in paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells cells using ab186735 at 1/90 dilution (red) and a rabbit IgG as negative control (green).

  • Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Immunohistochemistry (Frozen sections) analysis of mouse small intestine tissue sections labeling TOMM20 with Purified ab186735 at 1/50 (1.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
  • Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Immunohistochemistry (Frozen sections) analysis of mouse cardiac muscle tissue sections labeling TOMM20 with Purified ab186735 at 1/50 (1.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

    Paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized human non-small cell lung carcinoma cells labeleing TOMM20 using ab186735 at 1/400 dilution for 3 hours at room temperature. A Goat anti-Rabbit IgG (H&L) AlexaFluor® was used as secondary antibody at 1/1000 dilution.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

    Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling TOMM20 (green) with ab186735 at 1/250 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling TOMM20 with ab186735 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

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