Antibodies, Proteins, Kits and Reagents for Life Science

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Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker
Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
See all TOMM20 primary antibodies
Description

Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2 and HeLa whole cell lysate (ab150035). IHC-P: Human endometrial adenocarcinoma, human cervical cancer, mouse cardiac muscle tissues, rat testis, mouse colon. ICC/IF: HeLa cells. Flow Cyt: NCI-H2228 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15581-39
Isotype

IgG
Research areas

Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734) at 1/5000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 16 kDa
Observed band size: 16 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cells) labeling TOMM20 with ab186734 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/100 was used as counterstain antibody.

Confocal image showing cytoplasmic staining in HeLa cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

Granular cytoplasmic staining on human cervical cancer.
Flow Cytometry - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Flow cytometry analysis of TOMM20 expression in NCI-H2228 (Human lung cancer cell line) cells using ab186734 at 1/40 dilution (pink) and a rabbit IgG as negative control (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

Granular cytoplasmic staining on mouse colon.
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734) Samandi et al eLife. 2017; 6: e27860. Published online 2017 Oct 30. doi: 10.7554/eLife.27860

AltMiD51^5’ expression induces mitochondrial fission.

Confocal microscopy of mock-transfected cells, cells transfected with altMiD51^WT, altMiD51^LYR→AAA or Drp1^K38A immunostained with anti-TOM20 (red channel) and anti-Flag (green channel) monoclonal antibodies. In each image, boxed areas are shown at higher magnification in the bottom right corner.

TOMM20 is detected using ab186734 at 1/500 dilution.

From Figure 12c of Samandi et al.

Samandi et al eLife. 2017; 6: e27860. Published online 2017 Oct 30. doi: 10.7554/eLife.27860

Reproduced underthe Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, labeling TOMM20 (green) with ab186734 at 1/250 dilution.

Alexa Fluor^®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunohistochemical analysis of paraffin-embedded rat testis tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

Granular cytoplasmic staining on rat testis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunohistochemical analysis of paraffin-embedded human endometrial adenocarcinoma tissue, labeling TOMM20 with ab186734 at 1/100 dilution.

Detected using HRP polymer for Rabbit IgG and counterstained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling TOMM20 with ab186734 at 1/100 dilution.

Detected using HRP polymer for Rabbit IgG and counterstained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734) at 1/20000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 16 kDa
Observed band size: 16 kDa
Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

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