Anti-eIF2A antibody [3A7B11] (ab181467)
![Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-260193-anti-eif2a-antibody-3a7b11-western-blot.jpg "Anti-eIF2A antibody [3A7B11] (ab181467)")
Key features and details
- Mouse monoclonal [3A7B11] to eIF2A
- Suitable for: Flow Cyt, IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-eIF2A antibody [3A7B11]
See all eIF2A primary antibodies -
Description
Mouse monoclonal [3A7B11] to eIF2A -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human eIF2A aa 448-576. (Expressed in E.coli).
Sequence:PPALRNKPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRKHEAKKAA KQEARSDKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAI EQLKEQAATGKQLEKNQLEK IQKETALLQ
Database link: Q9BY44 -
Positive control
- Recombinant Human eIF2A (aa 448-576) protein; eIF2A (aa 448-576)-hIgGFc transfected HEK293 cell lysate; MCF7, PC-12, HepG2, HeLa, Cos7, K562, Jurkat, A431 and NIH3T3 cell lysates; HepG2 cells; Human cervical cancer and bladder cancer tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3A7B11 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: eIF2A knockout HAP1 cell lysate (20 µg)
Lane 3: Ramos cell lysate (20 µg)
Lane 4: MOLT4 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab181467 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab181467 was shown to recognize eIF2A when eIF2A knockout samples were used, along with additional cross-reactive bands. Wild-type and eIF2A knockout samples were subjected to SDS-PAGE. ab181467 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-210608-anti-eif2a-antibody-3a7b11-western-blot.jpg)
Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)All lanes : Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : COS-7 cell lysate
Lane 6 : K562 cell lysate
Lane 7 : Jurkat cell lysate
Lane 8 : A431 cell lysate
Lane 9 : NIH3T3 cell lysate
Predicted band size: 65 kDa
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![Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-210609-anti-eif2a-antibody-3a7b11-western-blot.jpg)
Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution + Recombinant Human eIF2A (aa 448-576) protein
Predicted band size: 65 kDaExpected MWt is 40.3 kDa.
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![Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-210610-anti-eif2a-antibody-3a7b11-western-blot.jpg)
Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)All lanes : Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution
Lane 1 : Non-transfected HEK293 cell lysate
Lane 2 : eIF2A (aa 448-576)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 65 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-210614-anti-eif2a-antibody-3a7b11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling eIF2A with ab181467 at 1/200 dilution with DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-210613-anti-eif2a-antibody-3a7b11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling eIF2A with ab181467 at 1/200 dilution with DAB staining.
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![Flow Cytometry - Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-210612-anti-eif2a-antibody-3a7b11-flow-cytometry.jpg)
Flow Cytometry - Anti-eIF2A antibody [3A7B11] (ab181467)Flow cytometric analysis of HepG2 cells labeling eIF2A with ab181467 at 1/200 dilution (green) compared to a negative control (red).
