Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-eIF2A antibody [EPR11041] (ab157478)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11041] to eIF2A
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-eIF2A antibody [EPR11041]
    See all eIF2A primary antibodies

  • Description

    Rabbit monoclonal [EPR11041] to eIF2A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human eIF2A. The exact sequence is proprietary.

  • Positive control

    • Ramos, MOLT4, U87-MG and Raji cell lysates; Raji cells. ICC/IF: HAP1 wildtype and HAP1-EIF2A knockout cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-eIF2A antibody [EPR11041] (ab157478)
    Western blot - Anti-eIF2A antibody [EPR11041] (ab157478)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: eIF2A knockout HAP1 cell lysate (20 µg)
    Lane 3: U87-MG cell lysate (20 µg)
    Lane 4: MOLT4 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab157478 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab157478 was shown to recognize eIF2A when eIF2A knockout samples were used, along with additional cross-reactive bands. Wild-type and eIF2A knockout samples were subjected to SDS-PAGE. ab157478 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF2A antibody [EPR11041] (ab157478)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF2A antibody [EPR11041] (ab157478)

    ab157478 staining eIF2A in wild-type HAP1 cells (top panel) and EIF2A knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol for 5 minutes, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab157478 at 1μg/ml and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1 hour with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

     

  • Western blot - Anti-eIF2A antibody [EPR11041] (ab157478)
    Western blot - Anti-eIF2A antibody [EPR11041] (ab157478)
    All lanes : Anti-eIF2A antibody [EPR11041] (ab157478) at 1/1000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : MOLT4 cell lysate
    Lane 3 : U87-MG cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 65 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF2A antibody [EPR11041] (ab157478)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF2A antibody [EPR11041] (ab157478)
    Immunofluorescent analysis of Raji cells labeling eIF2A with ab157478 at 1/100 dilution.
  • Anti-eIF2A antibody [EPR11041] (ab157478)
    Anti-eIF2A antibody [EPR11041] (ab157478)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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