HRP Anti-eIF2A antibody [EPR11042] (ab215342)
![HRP Anti-eIF2A antibody [EPR11042] (ab215342)](https://www.abcam.com/ps/products/215/ab215342/Images/ab215342-307582-ab215342HRPKOWBJRIZ.jpg "HRP Anti-eIF2A antibody [EPR11042] (ab215342)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR11042] to eIF2A
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-eIF2A antibody [EPR11042]
See all eIF2A primary antibodies -
Description
HRP Rabbit monoclonal [EPR11042] to eIF2A -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MOLT4, Ramos, HAP1 WT (shows pos.) and HAP1 eIF2A KO (shows neg.)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11042 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-eIF2A antibody [EPR11042] (ab215342)All lanes : HRP Anti-eIF2A antibody [EPR11042] (ab215342) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : EIF2A (eIF2A) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 8 minutesab215342 was shown to specifically react with eIF2A in wild-type HAP1 cells as signal was lost in EIF2A (eIF2A) knockout cells. Wild-type and EIF2A (eIF2A) knockout samples were subjected to SDS-PAGE. ab215342 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-eIF2A antibody [EPR11042] (ab215342)](https://www.abcam.com/ps/products/215/ab215342/Images/ab215342-283942-WBab2153421.jpg)
Western blot - HRP Anti-eIF2A antibody [EPR11042] (ab215342)All lanes : HRP Anti-eIF2A antibody [EPR11042] (ab215342) at 1/5000 dilution
Lane 1 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg
Lane 3 : HAP1 WT at 20 µg
Lane 4 : eIF2A knockout HAP1 at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215342 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-eIF2A antibody [EPR11042] (ab215342)](https://www.abcam.com/ps/products/215/ab215342/Images/ab215342-3-benefits-of-recombinant-antibodies.png)
HRP Anti-eIF2A antibody [EPR11042] (ab215342)
