Anti-eIF2A antibody (ab264254)
Key features and details
- Rabbit polyclonal to eIF2A
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF2A antibody
See all eIF2A primary antibodies -
Description
Rabbit polyclonal to eIF2A -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human eIF2A aa 525-575. The exact sequence is proprietary.
Database link: Q9BY44 -
Positive control
- IHC-P: Human Breast Carcinoma tissue. IP: HeLa whole cell lysate. WB: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF2A antibody (ab264254)All lanes : Anti-eIF2A antibody (ab264254) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Exposure time: 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody (ab264254)Immunohistochemical analysis of FFPE human breast carcinoma tissue section labelling eIF2A with ab264254 at 1/200 dilution. DAB was used a counterstain.
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Immunoprecipitation - Anti-eIF2A antibody (ab264254)eIF2A was immunoprecipitated from 1mg of HeLa whole cell lysates using eIF2A antibodies at 3μg/reaction. Western blot was performed on the immunprecipitates using ab264253 (lane 1), ab264254 (lane 2) and a control IgG (lane 3) at 1μg/ml.
Detected with chemilimuniscence with exposure time of 3 seconds.
