Anti-eIF2A antibody (ab264253)
Key features and details
- Rabbit polyclonal to eIF2A
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF2A antibody
See all eIF2A primary antibodies -
Description
Rabbit polyclonal to eIF2A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP Human
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Immunogen
Synthetic peptide within Human eIF2A aa 450-500. The exact sequence is proprietary. NP_114414.2
Database link: Q9BY44 -
Positive control
- IHC-P: Human Colon Carcinoma tissue. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-eIF2A antibody (ab264253)
eIF2A was immunoprecipitated from 1mg of HeLa whole cell lysates using eIF2A antibodies at 3μg/reaction. Western blot was performed on the immunprecipitates using ab264254 (lane 1), ab264253 (lane 2) and a control IgG (lane 3) at 1μg/ml.
Detected with chemilimuniscence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody (ab264253)Immunohistochemical analysis of FFPE human colon carcinoma tissue section labelling eIF2A with ab264233 at 1/500 dilution. DAB was used a counterstain.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
