Recombinant human TIGIT protein (Active) (ab224737)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, Flow Cyt, Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.40
Constituents: PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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SDS-PAGE - Recombinant human TIGIT protein (Active) (ab224737)
ab224737 analyzed by SDS-PAGE under reducing conditions and stained overnight with Coomassie Blue. The protein migrates as 17-21 kDa under reducing (+) condition due to glycosylation.
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Functional Studies - Recombinant human TIGIT protein (Active) (ab224737)Immobilized ab224737 at 2 μg/ml (100 μl/well) can bind Human CD155, Fc Tag with a linear range of 8-128 ng/ml.
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Functional Studies - Recombinant human TIGIT protein (Active) (ab224737)The purity of ab224737 was more than 90% and around 30-45 kDa verified by SEC-MALS.
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Functional Studies - Recombinant human TIGIT protein (Active) (ab224737)Immobilized ab224737 at 1 μg/ml (100 μl/well) can bind Human Nectin-3, Fc Tag with a linear range of 0.2-2.5 μg/mL.
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Functional Studies - Recombinant human TIGIT protein (Active) (ab224737)Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 µg/mL to 0.0097 µg/mL) were added into Human TIGIT, His Tag : Biotinylated Human CD155, Fc Tag binding reactions. Background was subtracted from data points before curve fitting. -
Functional Studies - Recombinant human TIGIT protein (Active) (ab224737)Anti-Human TIGIT MAb (Mouse IgG1) captured on CM5 chip via Anti-Mouse antibodies surface, can bind Human TIGIT, His Tag with an affinity constant of 3.93 nM as determined in a SPR assay (Biacore T200). -
Functional Studies - Recombinant human TIGIT protein (Active) (ab224737)Loaded Anti-Human TIGIT MAb (Mouse IgG1) on AMC Biosensor, can bind Recombinant human TIGIT protein (ab224737) with an affinity constant of 3.72 nM as determined in BLI assay.
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Functional Studies - Recombinant human TIGIT protein (Active) (ab224737)Loaded Recombinant human Poliovirus Receptor/PVR protein (ab220561) on Protein A Biosensor, can bind Recombinant human TIGIT protein (ab224737) with an affinity constant of 0.23 μM as determined in BLI assay.
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Functional Studies - Recombinant human TIGIT protein (Active) (ab224737)Loaded Recombinant human Nectin 3 protein (ab221336) on Protein A Biosensor, can bind Recombinant human TIGIT protein (ab224737) with an affinity constant of 0.732 μM as determined in BLI assay.
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Functional Studies - Recombinant human TIGIT protein (Active) (ab224737)FACS assay shows that Human TIGIT, His Tag can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.3 µg/mL.
