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Immunology Adaptive Immunity T Cells Non-CD

Anti-URP2/Kindlin-3 antibody [3D6] (ab173416)

Price and availability

281 433 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-URP2/Kindlin-3 antibody [3D6] (ab173416)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [3D6] to URP2/Kindlin-3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-URP2/Kindlin-3 antibody [3D6]
    See all URP2/Kindlin-3 primary antibodies
  • Description

    Mouse monoclonal [3D6] to URP2/Kindlin-3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human URP2/Kindlin-3. Bacterial expressed.

  • Epitope

    The epitope for clone 3D6 has been mapped to residues 181-461.
  • Positive control

    • This antibody gave a positive signal in human spleen tissue lysate and in the following whole cell lysates: Raji; K562; THP1. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal spleen.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Previously labelled as URP2. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    3D6
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Vascular
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-URP2/Kindlin-3 antibody [3D6] (ab173416)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-URP2/Kindlin-3 antibody [3D6] (ab173416)

    IHC image of URP2/Kindlin-3 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab173416, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-URP2/Kindlin-3 antibody [3D6] (ab173416)
    Western blot - Anti-URP2/Kindlin-3 antibody [3D6] (ab173416)
    All lanes : Anti-URP2/Kindlin-3 antibody [3D6] (ab173416) at 5 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 4 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 76 kDa
    Observed band size: 76 kDa
    Additional bands at: 33 kDa (possible cleavage fragment)


    Exposure time: 4 minutes


    We believe that the band observed at approximately 33-kDa is a C-terminal cleavage fragment of URP2/Kindlin-3, which has been described in the literature (PMID 23012377).    Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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