Anti-URP2/Kindlin-3 antibody (ab68040)
Key features and details
- Rabbit polyclonal to URP2/Kindlin-3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-URP2/Kindlin-3 antibody
See all URP2/Kindlin-3 primary antibodies -
Description
Rabbit polyclonal to URP2/Kindlin-3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
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General notes
Previously labelled as URP2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab68040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). Target
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Function
Plays a central role in cell adhesion in hematopoietic cells. Acts by activating the integrin beta-1-3 (ITGB1, ITGB2 and ITGB3). Required for integrin-mediated platelet adhesion and leukocyte adhesion to endothelial cells. Required for activation of integrin beta-2 (ITGB2) in polymorphonuclear granulocytes (PMNs).
Isoform 2 may act as a repressor of NF-kappa-B and apoptosis. -
Tissue specificity
Highly expressed in lymph node. Expressed in thymus, spleen and leukocytes. Weakly expressed in placenta, small intestine, stomach, testis and lung. Overexpressed in B-cell malignancies. -
Involvement in disease
Defects in FERMT3 are the cause of leukocyte adhesion deficiency type 3 (LAD3) [MIM:612840]; also called leukocyte adhesion deficiency 1 variant (LAD1v). LAD3 is a rare syndrome characterized by infections without pus formation in the presence of a leukocytosis combined with a Glanzmann-type bleeding disorder, resulting from a hematopoietic defect in integrin activation. Symptoms arise from an inability to activate the integrins expressed on hematopoietic cells, including platelets and leukocytes. -
Sequence similarities
Belongs to the kindlin family.
Contains 1 FERM domain.
Contains 1 PH domain. -
Domain
The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain. -
Cellular localization
Cell projection > podosome. Present in the F-actin surrounding ring structure of podosomes, which are specialized adhesion structures of hematopoietic cells. - Information by UniProt
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Database links
- Entrez Gene: 525159 Cow
- Entrez Gene: 83706 Human
- Entrez Gene: 108101 Mouse
- Entrez Gene: 309186 Rat
- Omim: 607901 Human
- SwissProt: Q32LP0 Cow
- SwissProt: Q86UX7 Human
- SwissProt: Q8K1B8 Mouse
see all -
Alternative names
- Fermitin family homolog 3 antibody
- Fermitin family member 3 antibody
- FERMT3 antibody
see all
Images
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Western blot - Anti-URP2/Kindlin-3 antibody (ab68040)All lanes : Anti-URP2/Kindlin-3 antibody (ab68040) at 1 µg/ml
Lane 1 : Kidney (Rat) Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lane 3 : Thymus (Rat) Tissue Lysate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 2 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-URP2/Kindlin-3 antibody (ab68040)IHC image of URP2/Kindlin-3 staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68040, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (5)
ab68040 has been referenced in 5 publications.
- Singh D et al. Cooperative Stimulation of Megakaryocytic Differentiation by Gfi1b Gene Targets Kindlin3 and Talin1. PLoS One 11:e0164506 (2016). WB ; Mouse . PubMed: 27768697
- Djaafri I et al. A novel tumor suppressor function of Kindlin-3 in solid cancer. Oncotarget 5:8970-85 (2014). IHC . PubMed: 25344860
- Canault M et al. Human CalDAG-GEFI gene (RASGRP2) mutation affects platelet function and causes severe bleeding. J Exp Med 211:1349-62 (2014). PubMed: 24958846
- Alonso-Orgaz S et al. Proteomic characterization of human coronary thrombus in patients with ST-segment elevation acute myocardial infarction. J Proteomics 109C:368-381 (2014). WB ; Human . PubMed: 25065646
- Dixit N et al. Leukocyte function antigen-1, kindlin-3, and calcium flux orchestrate neutrophil recruitment during inflammation. J Immunol 189:5954-64 (2012). WB ; Human . PubMed: 23144497
Images
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Western blot - Anti-URP2/Kindlin-3 antibody (ab68040)All lanes : Anti-URP2/Kindlin-3 antibody (ab68040) at 1 µg/ml
Lane 1 : Kidney (Rat) Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lane 3 : Thymus (Rat) Tissue Lysate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 2 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-URP2/Kindlin-3 antibody (ab68040)
IHC image of URP2/Kindlin-3 staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68040, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
