Human IL-4 ELISA Kit High Sensitivity (ab46063)
Key features and details
- Sensitivity: 0.31 pg/ml
- Range: 0.31 pg/ml - 10 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-4 ELISA Kit High Sensitivity
See all IL-4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% A 8 7.43pg/ml 0.9 12.3% B 8 6.2pg/ml 0.7 10.8% C 8 8.77pg/ml 0.7 7.6% D 8 7.36pg/ml 0.4 6% Inter-assay Sample n Mean SD CV% A 9 7.1pg/ml 0.83 11.8% B 9 6.47pg/ml 0.72 11.1% C 9 8.76pg/ml 1.37 15.7% D 9 7.44pg/ml 1.2 16.1% E 7 2pg/ml 0.874 17% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.31 pg/ml -
Range
0.31 pg/ml - 10 pg/ml -
Recovery
101 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 97% - 127% Serum 69 61% - 75% -
Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-4 Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in Human serum, plasma, buffered solution and cell culture media.
A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-4.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 200X Wash Buffer White 1 x 10ml Amplification Diluent Brown & blue spot 1 x 25ml Amplifier Yellow 1 x 200µl Biotinylated anti IL-4 1 x 400µl Biotinylated Antibody Diluent Red 1 x 7.5ml Chromogen TMB Substrate Solution 1 x 11ml HRP Diluent Red 1 x 25ml IL-4 Microplate (12 x 8 well strips) 1 unit IL-4 Standard (Lyophilized) 2 vials Stop Reagent Black 1 x 11ml Streptavidin-HRP 2 x 5µl -
Research areas
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Relevance
IL-4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL-4. IL-4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL-4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL-4 encoding distinct isoforms have been reported. -
Cellular localization
Secreted -
Alternative names
- B cell growth factor 1
- B cell stimulatory factor 1
- BCGF 1
see all -
Database links
- Entrez Gene: 3565 Human
- Omim: 147780 Human
- SwissProt: P05112 Human
Images
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ELISA: IL-4 High Sensitivity Human ELISA Kit (ab46063)
Standard curve of human IL-4, with background signal subtracted (duplicates; +/- SD).
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ELISA: IL-4 High Sensitivity Human ELISA Kit (ab46063)IL-4 measured in cell culture supernatants from control (PBMC) or treated (PBMC+PHA; 2% PHA-M; LifeTechnologies) cells, grown for 48 hours (duplicates +/- SD). Samples tested neat - 1/10, control supernatant gave signals below level of detection.
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Typical Standard CurveRepresentative Standard Curve using ab46063
