Human IL-4 ELISA Kit (ab46058)
Key features and details
- Sensitivity: 0.5 pg/ml
- Range: 1.1 pg/ml - 35 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated anti IL-4 1 x 400µl 2 x 400µl Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Control 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml IL-4 Microplate (12 x 8 well strips) 1 unit 2 units IL-4 Standard (Lyophilized) 2 vials 4 vials Standard Diluent (Serum) 1 x 7ml 2 x 7ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
Research areas
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Relevance
IL-4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL-4. IL-4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL-4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL-4 encoding distinct isoforms have been reported. -
Cellular localization
Secreted -
Alternative names
- B cell growth factor 1
- B cell stimulatory factor 1
- BCGF 1
see all -
Database links
- Entrez Gene: 3565 Human
- Omim: 147780 Human
- SwissProt: P05112 Human
Images
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Typical data
Representative standard curve using ab46058
