Mouse IL-5 ELISA Kit (ab204523)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.2 pg/ml
- Range: 3.9 pg/ml - 250 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-5 ELISA Kit
See all IL-5 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 3.3% Inter-assay Sample n Mean SD CV% Overall 3 5.1% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.2 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 104% - 107% Serum 105 101% - 107% Hep Plasma 88 85% - 91% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Sheep, Goat -
Product overview
Mouse IL-5 ELISA Kit (ab204523) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-5 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-5 with 1.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin 5 (IL-5) is a small, 113 amino acid cytokine produced by T cells involved in the differentiation of B-cells to immunoglobulin secreting cells. IL-5 is a key mediator in the survival and effector functions of mature eosinophils. Due to its effects on eosinophils, IL-5 is implicated in the pathogenesis of asthma and other hypereosinophilic inflammatory conditions.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IL-5 Capture Antibody 1 x 600µl 10X Mouse IL-5 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Mouse IL-5 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. -
Sequence similarities
Belongs to the IL-5 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- B-cell differentiation factor I
- Colony stimulating factor
- EDF
see all -
Database links
- Entrez Gene: 16191 Mouse
- SwissProt: P04401 Mouse
- Unigene: 4461 Mouse
Images
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Other - Mouse IL-5 ELISA Kit (ab204523)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse IL-5 standard curve comparison data.Standard curve comparison between mouse IL-5 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 5-fold increase in sensitivity.
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Example of IL-5 standard curve prepared in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of IL-5 standard curve prepared in Sample Diluent 25BP.Background-subtracted data values (mean +/- SD) are graphed.
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Linearity of dilution of the assay.Interpolated concentrations of IL-5 in mouse cell culture media. The concentrations of IL-5 were measured in duplicate and interpolated from the IL-5 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 concentration was determined to be 642 pg/mL in mouse EL-4 (IL-2) PHA/PMA-stimulated supernatant.
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Comparison of secreted Il-5 in unstimulated and PHA/PMA stimulated EL-4 (IL-2) Cells.EL-4 (IL-2) cells were grown in the absence (unstimulated) or presence of 10 ug/mL PHA and 10 ng/mL PMA (stimulated) for 48 hours. IL-5 was measured in undiluted cell culture supernatants of unstimulated and PHA/PMA stimulated EL-4 (IL-2). Measured values were interpolated from the IL-5 Standard Curve diluted in Sample Diluent NS. Mean of duplicate values +/-SD are graphed: 1.05 pg/mL unstimulated, 535 pg/mL stimulated.
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Sandwich ELISA - Mouse IL-5 ELISA Kit (ab204523)To learn more about the advantages of recombinant antibodies see here.
