Mouse gp130 ELISA Kit (ab193724)
Key features and details
- Sensitivity: 40 pg/ml
- Range: 40 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse gp130 ELISA Kit
See all CD130 (gp130) kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
40 pg/ml -
Range
40 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99.02 91% - 107% Plasma 97.9 90% - 103% Cell culture media 118.7 113% - 123% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam's gp130 MouseELISA Kit (ab193724) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse gp130 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for gp130 coated onto a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures gp130 present in the sample. The wells are washed and a biotinylated anti-Mouse gp130 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of gp130 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 350X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated mouse gp-130 detection antibody (lyophilized) 2 vials mouse gp-130 standards (lyophilized) 2 vials Pre-coated mouse gp-130 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
CD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. -
Cellular localization
Cell membrane; single-pass type I membrane protein and secreted -
Alternative names
- CD130 antigen
- CDw130
- gp130
see all -
Database links
- Entrez Gene: 16195 Mouse
- SwissProt: Q6PDI9 Mouse
Images
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Standard Curve.
Typical Standard Curve in Assay Diluent A using ab193724 gp130 Mouse ELISA Kit.
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Standard Curve.Typical Standard Curve in Assay Diluent B using ab193724 gp130 Mouse ELISA Kit.
