Human TNF beta ELISA Kit (ab229202)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.84 pg/ml
- Range: 16 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Rhesus monkey
Overview
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Product name
Human TNF beta ELISA Kit
See all LTA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% PBMC s/n 9 2.5% Inter-assay Sample n Mean SD CV% PBMC s/n 3 3.3% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.84 pg/ml -
Range
16 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 101 98% - 103% Serum 82 82% - 83% Hep Plasma 87 87% - 89% EDTA Plasma 81 80% - 82% Cit plasma 80 79% - 81% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human, Rhesus monkey -
Product overview
Human TNF beta ELISA Kit (ab229202) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TNF beta protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TNF beta with 1.84 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TNF beta Capture Antibody 1 x 600µl 10X Human TNF beta Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Human TNF beta Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. -
Involvement in disease
Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated. - Information by UniProt
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Alternative names
- LTalpha
- DAMA-25N12.13-004
- DIF
see all -
Database links
- Entrez Gene: 4049 Human
- Entrez Gene: 715425 Rhesus monkey
- Omim: 153440 Human
- SwissProt: P01374 Human
- SwissProt: Q5TM20 Rhesus monkey
- Unigene: 36 Human
Images
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Other - Human TNF beta ELISA Kit (ab229202)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human TNF beta standard curve in Sample Diluent NS.The TNF beta standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked TNF beta in human serum and plasma samples.The concentrations of TNF beta were measured in duplicates, interpolated from the TNF beta standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (heparin) 25% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native TNF beta in human and Rhesus Macaque PBMC cell culture supernatants.PBMCs were either stimulated with 1.5% PHA or unstimulated for 48 hours. The concentrations of TNF beta were measured in duplicate and interpolated from the TNF beta standard curve and corrected for sample dilution. Undiluted samples are as follows: Stimulated human PBMC supernatant 25% and stimulated Rhesus Macaque PBMC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF beta concentration was determined to be 1,660 pg/mL in the stimulated human PBMC supernatant, 1,940 pg/mL in the Rhesus Macaque stimulated PBMC supernatant, and undetectable in the unstimulated human PBMC supernatant (not shown).
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Sandwich ELISA - Human TNF beta ELISA Kit (ab229202)To learn more about the advantages of recombinant antibodies see here.
