Urea Assay Kit II (ab234052)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Cell Lysate, Milk, Plasma, Serum, Tissue Extracts, Urine
Overview
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Product name
Urea Assay Kit II
See all Urea kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Plasma, Tissue Extracts, Cell Lysate -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Urea Assay Kit II (ab234052) is a fast, sensitive and easy to use kit that measures urea level in various tissues/cells. It is based on Jung’s method with a modification that delivers more robust and sensitive data. The urea is condensed with o-phthalaldehyde (OPA), followed by a reaction to form a colored product with strong absorbance at 505 nm. It can measure less than 10 μM urea in 96-well plate assay.
This urea assay kit provides an alternative to the most popular urea assay kit ab83362, and uses a different assay method.
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Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Urea Reagent A 1 x 12ml Urea Reagent B 1 x 12ml Urea Standard (100 mM) 1 x 100µl -
Research areas
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Relevance
Urea is a waste product which is produced in the liver, dissolved in blood (in a concentration of 2.5 - 7.5 mM), and secreted by the kidneys. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body, and the countercurrent system which allows for reabsorption of water and critical ions in the nephrons. -
Alternative names
- Carbamide
- Carbonyl diamide
- Carbonyldiamine
Images
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Urea Standard Curve.
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Measurement of urea in human urine (5 µL of 50 times diluted), human serum (5 µL) and 2% milk (20 µL).
