Urea Assay Kit (ab83362)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media, Cell Lysate, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.5 nmol/well
Overview
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Product name
Urea Assay Kit
See all Urea kits -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Quantitative -
Sensitivity
> 0.5 nmol/well -
Range
0.5 nmol/well - 5 nmol/well -
Assay time
1h 00m -
Product overview
Urea Assay Kit ab83362 is a rapid, simple, sensitive, and reliable assay used to quantify urea in a variety of samples such as serum, plasma, and urine, etc. It can be used as a blood urea nitrogen assay kit (BUN assay kit).
In the urea assay protocol, urea is acted on by enzymes to form a product that reacts with a probe to generate color (ODmax=570nm). The absorbance is directly proportional to the concentration of urea in the solution. The kit can detect as low as 0.5 nmol per well or 10 µM of urea.
Urea assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 60 min at 37ºC
- analyze with microplate reader -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Converting Enzyme (Lyophilised) Blue 1 vial Developer (Lyophilised) Orange 1 vial Enzyme Mix (lyophilized) Green 1 vial OxiRed Probe in DMSO Red 1 x 200µl Urea Assay Buffer WM 1 x 25ml Urea Standard (100mM) Yellow 1 x 100µl -
Research areas
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Relevance
Urea is a waste product which is produced in the liver, dissolved in blood (in a concentration of 2.5 - 7.5 mM), and secreted by the kidneys. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body, and the countercurrent system which allows for reabsorption of water and critical ions in the nephrons. -
Alternative names
- Carbamide
- Carbonyl diamide
- Carbonyldiamine
Images
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Functional Studies - Urea Assay Kit (ab83362) Howie, R Nicole et al., PloS one?vol. 10,7 e0132520., Fig 6, doi:10.1371/journal.pone.0132520
ab83362 was used as a BUN assay (Blood urea nitrogen assay) to measure urea levels in 80 female Sprague-Dawley rats (age 10-12 months-old).
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Functional Studies - Urea Assay Kit (ab83362)Urea measured in cell culture medium and control medium (MEM), background signal subtracted (duplicates +/- SD).
The samples were tested neat; 1:4; 1:16 and 1:64. Only the latter two gave reliable results.
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Functional Studies - Urea Assay Kit (ab83362)Urea measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).
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Functional Studies - Urea Assay Kit (ab83362)Urea measured in cell lysates, background signal subtracted (duplicates; +/- SD).
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Functional Studies - Urea Assay Kit (ab83362)Urea measured in biological fluids, background signal subtracted (duplicates; +/- SD).
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Functional Studies - Urea Assay Kit (ab83362)Example of Urea standard curve using ab83362
