Anti-Ref2P antibody (ab178440)
Key features and details
- Rabbit polyclonal to Ref2P
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-Ref2P antibody -
Description
Rabbit polyclonal to Ref2P -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
Synthetic peptide corresponding to Drosophila melanogaster Ref2P aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: P14199 -
Positive control
- This antibody gave a positive signal in Atg8a mutant Fruit Flies (D.melanogaster).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab178440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use at an assay dependent concentration. Predicted molecular weight: 65 kDa. Target
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Function
Implicated in sigma rhabdovirus multiplication and necessary for male fertility. -
Sequence similarities
Contains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 35246 Drosophila melanogaster
- SwissProt: P14199 Drosophila melanogaster
- Unigene: 12210 Drosophila melanogaster
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Alternative names
- Protein ref(2)P antibody
- ref(2)P antibody
- REF2P_DROME antibody
- Refractory to sigma P antibody
Images
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Western blot - Anti-Ref2P antibody (ab178440)This image is courtesy of Dr Anne-Claire Jacomin / Ioannis Nezis Lab, University of WarwickAll lanes : Anti-Ref2P antibody (ab178440) at 1/500 dilution (Blocking 60 minutes in TBS+0.1% Tween-20 + 5% non-fat milk)
Lane 1 : Wt adult drosophila lysate
Lane 2 : Atg8a mutant adult drosophila lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit HRP at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsGel 8% acrylamide, transferred onto PVDF membrane for 60 minutes at 100V in 10% ethanol buffer. Anti-Ref2P antibody 1:500 incubated overnight at 4ºC, with gentle agitation in TBS + 0.1% Tween-20.
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Western blot - Anti-Ref2P antibody (ab178440)All lanes : Anti-Ref2P antibody (ab178440) at 1/100 dilution (Milk blocked)
Lanes 1-3 : Atg8a mutant fruit flies (D.melanogaster)
Lanes 4-6 : Fruit flies (D.melanogaster) expressing ubiquitously Ref(2)p RNAi
Predicted band size: 65 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?The bands observed at the bottom of the gel represent Tubulin and were used as a loading control within this experiment.
Datasheets and documents
References (17)
ab178440 has been referenced in 17 publications.
- Brooks D et al. Drosophila NUAK functions with Starvin/BAG3 in autophagic protein turnover. PLoS Genet 16:e1008700 (2020). PubMed: 32320396
- Wen X et al. Tau Accumulation via Reduced Autophagy Mediates GGGGCC Repeat Expansion-Induced Neurodegeneration in Drosophila Model of ALS. Neurosci Bull N/A:N/A (2020). PubMed: 32500377
- Kanca O et al. An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms. Elife 8:N/A (2019). PubMed: 31674908
- Yan C et al. IRE1 promotes neurodegeneration through autophagy-dependent neuron death in the Drosophila model of Parkinson's disease. Cell Death Dis 10:800 (2019). PubMed: 31641108
- Jacomin AC et al. Impact of Autophagy and Aging on Iron Load and Ferritin in Drosophila Brain. Front Cell Dev Biol 7:142 (2019). PubMed: 31404236
Images
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Western blot - Anti-Ref2P antibody (ab178440) This image is courtesy of Dr Anne-Claire Jacomin / Ioannis Nezis Lab, University of WarwickAll lanes : Anti-Ref2P antibody (ab178440) at 1/500 dilution (Blocking 60 minutes in TBS+0.1% Tween-20 + 5% non-fat milk)
Lane 1 : Wt adult drosophila lysate
Lane 2 : Atg8a mutant adult drosophila lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit HRP at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsGel 8% acrylamide, transferred onto PVDF membrane for 60 minutes at 100V in 10% ethanol buffer. Anti-Ref2P antibody 1:500 incubated overnight at 4ºC, with gentle agitation in TBS + 0.1% Tween-20.
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Western blot - Anti-Ref2P antibody (ab178440)All lanes : Anti-Ref2P antibody (ab178440) at 1/100 dilution (Milk blocked)
Lanes 1-3 : Atg8a mutant fruit flies (D.melanogaster)
Lanes 4-6 : Fruit flies (D.melanogaster) expressing ubiquitously Ref(2)p RNAi
Predicted band size: 65 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?The bands observed at the bottom of the gel represent Tubulin and were used as a loading control within this experiment.