Complex IV Rodent Immunocapture Kit (ab109860)
Overview
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Product name
Complex IV Rodent Immunocapture Kit
See all Complex IV kits -
Species reactivity
Reacts with: Mouse, Rat -
Product overview
Complex IV Rodent Immunocapture Kit ab109860 allows for isolation of the cytochrome c oxidase complex from small amounts of tissue.
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.
This kit facilitates the analysis of both assembly state and accrued post-translational modifications of all 13 subunits of the cytochrome c oxidase complex. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109860 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
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Notes
Related products
Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 250 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads 1 x 250µg -
Research areas
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Alternative names
- cco
- COX
- Cytochrome c oxidase
- Cytochrome oxidase
