ab109801 Complex IV Immunocapture Kit allows isolation of the cytochrome c oxidase complex from small amounts of tissue.

250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.

This kit facilitates subsequent analysis of both assembly state and accrued post-translational modifications of all 13 subunits of Complex IV. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109801 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.

Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

• Signal Transduction
• Metabolism
• Mitochondrial

• Kits/ Lysates/ Other
• Kits
• Cell Metabolism Kits
• Other Metabolism Assay

• Metabolism
• Pathways and Processes
• Mitochondrial Metabolism
• Mitochondrial markers

• Metabolism
• Pathways and Processes
• Mitochondrial Metabolism
• Cytochromes

• Kits/ Lysates/ Other
• Kits
• Immunocapture
• OXPHOS