MitoTox™ Complex IV OXPHOS Activity Assay Kit (ab109906) is designed for testing the direct inhibitory effect of compounds on Complex IV activity in only 5 hours. Complex IV extracted from the provided bovine heart mitochondria (a rich source of Complex IV) is immunocaptured by specific antibodies on the plate. Complex IV activity can be observed as decrease in absorbance at OD 550 nm. The intra-assay and inter-assay variation of this assay are both

Inhibitory effects of compounds on Complex IV activity can be tested in two different ways: 1. Screening format, where up to 23 compounds can be tested at a single concentration in triplicate; 2. Dose response (IC₅₀) format, where two compounds known to affect Complex IV activity can be tested at 11 different data points in triplicate.

Testing for mitochondrial function has become a key aspect of drug discovery. Mitochondria can be affected by drug treatment, resulting into cardio- and hepatotoxic side effects that can lead to drug withdrawal from the market. Therefore, there is increasing emphasis on testing the impact on mitochondria early on in the drug development process to reduce failure rates during preclinical and clinical phases. 

• Signal Transduction
• Metabolism
• Mitochondrial

• Kits/ Lysates/ Other
• Kits
• Cell Metabolism Kits
• Other Metabolism Assay

• Metabolism
• Pathways and Processes
• Mitochondrial Metabolism
• Mitochondrial markers

• Metabolism
• Pathways and Processes
• Mitochondrial Metabolism
• Cytochromes