Anti-TTC11/FIS1 antibody (ab229969)
Key features and details
- Rabbit polyclonal to TTC11/FIS1
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TTC11/FIS1 antibody
See all TTC11/FIS1 primary antibodies -
Description
Rabbit polyclonal to TTC11/FIS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human TTC11/FIS1 aa 1-150.
Database link: Q9Y3D6 -
Positive control
- WB: SH-SY5Y whole cell lysate; Mouse heart and brain lysates. IHC-P: Human kidney tissue. ICC/IF: HeLa cells. IP: MCF7 whole cell lysate.
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General notes
Previously labelled as TTC11.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), 1% PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TTC11/FIS1 antibody (ab229969)All lanes : Anti-TTC11/FIS1 antibody (ab229969) at 1/1000 dilution
Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-TTC11/FIS1 antibody (ab229969)Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TTC11/FIS1 (green) using ab229969 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC11/FIS1 antibody (ab229969)Paraffin-embedded human kidney tissue stained for TTC11/FIS1 using ab229969 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunoprecipitation - Anti-TTC11/FIS1 antibody (ab229969)TTC11/FIS1 was immunoprecipitated from 500 µg MCF7 (human breast adenocarcinoma cell line) whole cell lysate with 4 µg of ab229969.
Lane 1: Rabbit monoclonal IgG (1 µg) IP instead of ab229969 in MCF7 whole cell lysate.
Lane 2: ab229969 IP in MCF whole cell lysate.
Lane 3: MCF7 whole cell lysate 20 ug (input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
