Human TNF beta ELISA Kit (ab119561)
Key features and details
- Sensitivity: 4.6 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TNF beta ELISA Kit
See all LTA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3 8% Inter-assay Sample n Mean SD CV% Overall 3 10.2% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.6 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
105 %
Sample specific recovery Sample type Average % Range Serum 105 87% - 130% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s TNF beta Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human TNF beta concentrations in cell culture supernatant, serum and plasma.
TNF beta specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with HRP-conjugated TNF beta detection antibody and the microplate is then incubated at room temperature and unbound conjugates are then washed away using wash buffer. TMB is the added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the TNF beta amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 2 units HRP-Conjugate anti-human TNF beta monoclonal antibody 1 x 200µl Microplate coated with monoclonal antibody to Human TNF beta (12 x 8 wells) 1 unit Sample Diluent 1 x 5ml Stop Solution (1M Phosphoric acid) 1 x 15ml TMB Substrate Solution 1 x 15ml TNF beta Standard 2 x 50µl -
Research areas
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Function
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. -
Involvement in disease
Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated. - Information by UniProt
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Alternative names
- LTalpha
- DAMA-25N12.13-004
- DIF
see all -
Database links
- Entrez Gene: 4049 Human
- Omim: 153440 Human
- SwissProt: P01374 Human
- Unigene: 36 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab119561.
