Anti-LEAP2 antibody (ab231955)
Key features and details
- Rabbit polyclonal to LEAP2
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-LEAP2 antibody -
Description
Rabbit polyclonal to LEAP2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Cow, Rhesus monkey
-
Immunogen
Recombinant fragment corresponding to Human LEAP2 aa 1 to the C-terminus. N-terminal His-tag and GST-tag (Expressed in E.coli).
Database link: Q969E1 -
Positive control
- WB: Pig liver and skeletal muscle tissues; Recombinant human LEAP2 protein containing a GST tag. IHC-P: Human prostate gland and rectum tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEAP2 antibody (ab231955)
Formalin-fixed, paraffin-embedded human rectum tissue stained for LEAP2 using ab231955 at 10 µg/ml in immunohistochemical analysis. DAB staining.
-
Western blot - Anti-LEAP2 antibody (ab231955)Anti-LEAP2 antibody (ab231955) at 2 µg/ml + Recombinant human LEAP2 protein containing a GST tag
Observed band size: 39 kDa why is the actual band size different from the predicted?
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEAP2 antibody (ab231955)Formalin-fixed, paraffin-embedded human prostate gland tissue stained for LEAP2 using ab231955 at 10 µg/ml in immunohistochemical analysis. DAB staining.
-
Western blot - Anti-LEAP2 antibody (ab231955)All lanes : Anti-LEAP2 antibody (ab231955) at 3 µg/ml
Lane 1 : Pig liver tissue lysate
Lane 2 : Pig skeletal muscle tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution