Anti-PARP1 antibody (ab75757)
Key features and details
- Chicken polyclonal to PARP1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgY
Overview
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Product name
Anti-PARP1 antibody
See all PARP1 primary antibodies -
Description
Chicken polyclonal to PARP1 -
Host species
Chicken -
Specificity
In WB, ab75757 recognizes intact PARP1 (113kDa), the 89kDa apoptosis-induced cleavage product, and other lower molecular weight fragments of PARP1. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Recombinant full length protein corresponding to Human PARP1. Highly purified human PARP1 expressed in baculovirus.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 0.316% Tris HCl -
Concentration information loading... -
Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Western blot - Anti-PARP1 antibody (ab75757)All lanes : Anti-PARP1 antibody (ab75757) at 1/1000 dilution
Lane 1 : Extract of HeLa cells treated with vehicle
Lane 2 : Extract of HeLa cells treated with staurosporine
Lane 3 : Extract of Jurkat cells treated with vehicle
Lane 4 : Extract of Jurkat cells treated with staurosporine
Lane 5 : Extract of C6 cells treated with vehicle
Lane 6 : Extract of C6 cells treated with camptothecin
Lane 7 : Extract of NIH 3T3 cells treated with vehicle
Lane 8 : Extract of NIH 3T3 cells treated with staurosporine
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-chicken IgY at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
Exposure time: 1 minuteSDS PAGE performed under reducing conditions (100mM DTT, Sample heated at 50°C).
Blocking: in 5% Milk + PBS for 3 hours at RT.
Primary antibody diluted in 5% Milk + PBS and incubated overnight at 4°C.
Secondary antibody diluted in 5% Milk + PBS and incubated for 2 hour at RT.
Predicted band size : 113 kDa: 89 kDa and 24 kDa.
Observed band size : 113 kDa: 89 kDa and 24 kDa. -
Western blot - Anti-PARP1 antibody (ab75757)All lanes : Anti-PARP1 antibody (ab75757) at 1 µg/ml
Lane 1 : Jurkat cell lysate at 5 µg
Lane 2 : Apoptotic Jurkat
cell lysate, induced with camptothecin for 4 hr
Secondary
All lanes : GAC-HRP at 1/2000 dilution
Predicted band size: 113 kDa
Additional bands at: 89 kDa (possible cleavage fragment)Note other smaller apoptosis-induced PARP1 fragments in Lane 2.
