Anti-HMGA2 antibody (ab97276)
Key features and details
- Rabbit polyclonal to HMGA2
- Suitable for: IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HMGA2 antibody
See all HMGA2 primary antibodies -
Description
Rabbit polyclonal to HMGA2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseRatHumanIP HumanWB MouseRatHuman
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Immunogen
Recombinant fragment corresponding to Human HMGA2 aa 1-93 (N terminal).
Database link: P52926 -
Positive control
- WB: MOLT-4, HepG2, HCT116, HeLa, A431, NIH/3T3, Raw264.7, C2C12, PC-12, Rat2 whole cell lysate. IP: HepG2 cells. ICC/IF: MCF7, HeLa cells. IHC-P: Rat brain, rat duodenum, human HuCCT1 xenograft, C2C12 xenograft.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilution
Lane 1 : A431 whole cell extract
Lane 2 : HeLa whole cell extract
Lane 3 : HepG2 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)Paraffin embedded C2C12 xenograft tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)HeLa cells stained for HMGA2 (green) using ab97276 at 1/500 dilution in ICC/IF.
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/1000 dilution
Lane 1 : NIH/3T3 whole cell extract
Lane 2 : Raw264.7 whole cell extract
Lane 3 : C2C12 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)Paraffin embedded human HuCCT1 xenograft tissue stained for HMGA2 using ab97276 at 1/250 dilution in immunohistochemical analysis.
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/1000 dilution
Lane 1 : PC-12 whole cell extract
Lane 2 : Rat2 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)Paraffin embedded rat duodenum tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-HMGA2 antibody (ab97276)Anti-HMGA2 antibody (ab97276) at 1/10000 dilution + HCT116 whole cell extract at 30 µg
Predicted band size: 12 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)Paraffin embedded rat brain tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-HMGA2 antibody (ab97276)Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody. Western blot analysis was performed using HMGA2 antibody at 1/500 dilution. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Input
Lane 2: Immunoprecipitation using control IgG
Lane 3: Immunoprecipitation using 5 μg ab97276
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Western blot - Anti-HMGA2 antibody (ab97276)Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + MOLT-4 whole cell lysate at 30 µg
Predicted band size: 12 kDa
15% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)ICC/IF image of ab97276 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilution
Lane 1 : HepG2
Lane 2 : HCT116
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa
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Western blot - Anti-HMGA2 antibody (ab97276) This image is courtesy of an anonymous abreview.Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + HeLa whole cell lysate at 25 µg
Secondary
Goat anti rabbit secondary IRDye 800CW at 1/1000 dilution
Predicted band size: 12 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
