Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)
![Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)](https://www.abcam.com/ps/products/248/ab248429/Images/ab248429-403338-anti-brg1-antibody-epncir111b-bsa-and-azide-free-western-blot.jpg "Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR111B] to BRG1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free
See all BRG1 primary antibodies -
Description
Rabbit monoclonal [EPNCIR111B] to BRG1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Mouse BRG1. The exact sequence is proprietary.
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General notes
ab248429 is the carrier-free version of ab133257 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248429 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPNCIR111B -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)
This data was developed using ab133257, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: BRG1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133257 observed at 240 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab133257 was shown to recognize BRG1 when BRG1 knockout samples were used, along with additional cross-reactive bands. Wild-type and BRG1 knockout samples were subjected to SDS-PAGE. ab133257 and ab18058 (loading control to vinculin) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)](https://www.abcam.com/ps/products/248/ab248429/Images/ab248429-399154-anti-brg1-antibody-epncir111b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)All lanes : Anti-BRG1 antibody [EPNCIR111B] (ab133257) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Molt 4 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody. at 1/2000 dilution
Predicted band size: 185 kDaThis data was developed using ab133257, the same antibody clone in a different buffer formulation.
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![Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)](https://www.abcam.com/ps/products/248/ab248429/Images/ab248429-3-benefits-of-recombinant-antibodies.png)
Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)
