Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR111B] to BRG1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free
    See all BRG1 primary antibodies

  • Description

    Rabbit monoclonal [EPNCIR111B] to BRG1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Mouse BRG1. The exact sequence is proprietary.

  • General notes

    ab248429 is the carrier-free version of ab133257 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248429 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.


    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)
    Western blot - Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)

    This data was developed using ab133257, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: BRG1 knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: K562 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133257 observed at 240 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab133257 was shown to recognize BRG1 when BRG1 knockout samples were used, along with additional cross-reactive bands. Wild-type and BRG1 knockout samples were subjected to SDS-PAGE. ab133257 and ab18058 (loading control to vinculin) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)
    Western blot - Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)
    All lanes : Anti-BRG1 antibody [EPNCIR111B] (ab133257) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Molt 4 cell lysate
    Lane 4 : PC12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody. at 1/2000 dilution

    Predicted band size: 185 kDa



    This data was developed using ab133257, the same antibody clone in a different buffer formulation.

     

  • Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)
    Anti-BRG1 antibody [EPNCIR111B] - BSA and Azide free (ab248429)

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