Anti-BRG1 antibody (ab4081)
Key features and details
- Rabbit polyclonal to BRG1
- Suitable for: WB
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-BRG1 antibody
See all BRG1 primary antibodies -
Description
Rabbit polyclonal to BRG1 -
Host species
Rabbit -
Specificity
We have conflicting data about the performance of this antibody in ChIP. Publications have reported positive results with this antibody using ChIP application, however we also have customer data indicating certain batches of this antibody did not work in ChIP in their hands. If you require any further information or assistance please contact Abcam Scientific Support Team. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human BRG1 aa 1400-1500 conjugated to keyhole limpet haemocyanin.
(Peptide available asab13736)
Images
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Western blot - Anti-BRG1 antibody (ab4081)All lanes : Anti-BRG1 antibody (ab4081) at 1/500 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : BRG1 knockout HAP1 cell lysate
Lane 3 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 185 kDaLanes 1 - 4: Merged signal (red and green).
Green - ab4081 observed at 240 kDa.
Red - loading control, Mouse monoclonal Anti-Vinculin antibody, observed at 124 kDa.
ab4081 was shown to recognize BRG1 when BRG1 knockout samples were used, along with additional cross-reactive bands.
Wild-type and BRG1 knockout samples were subjected to SDS-PAGE. Ab4081 and loading control to Vinculin were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773 - Green) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776 - Red) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-BRG1 antibody (ab4081)All lanes : Anti-BRG1 antibody (ab4081) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Jurkat nuclear extract lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 :Hep G2 nuclear extract lysate (ab14660)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 185 kDa
Observed band size: 235 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-BRG1 antibody (ab4081)All lanes : Anti-BRG1 antibody (ab4081) at 1/500 dilution
Lane 1 : HeLa (Human epithelial adenocarcinoma cell line) Nuclear extract
Lane 2 : HeLa Nuclear extract with Human BRG1 peptide (ab13736) at 1 µg
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) (Goat anti-rabbit (HRP))
Predicted band size: 185 kDa
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Western blot - Anti-BRG1 antibody (ab4081)Anti-BRG1 antibody (ab4081) at 1/1000 dilution +Recombinant human BRG1 protein (ab82237) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 185 kDa
Exposure time: 8 minutes -
Western blot - Anti-BRG1 antibody (ab4081) This image is courtesy of an anonymous abreview.Whole cell lysate prepared from HeLa cells was loaded at 150000 cells.
ab4081 used at a 1/1000 dilution.The secondary used was an HRP conjugated donkey polyclonal used at a 1/5000 dilution.
