Anti-BRG1 antibody [EPR3913] (ab92496)
![Anti-BRG1 antibody [EPR3913] (ab92496)](https://www.abcam.com/ps/products/92/ab92496/Images/ab92496-266695-anti-brg1-antibody-epr3913-western-blot.jpg "Anti-BRG1 antibody [EPR3913] (ab92496)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3913] to BRG1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-BRG1 antibody [EPR3913]
See all BRG1 primary antibodies -
Description
Rabbit monoclonal [EPR3913] to BRG1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human BRG1 aa 1550-1650 (C terminal). The exact sequence is proprietary.
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Positive control
- K562 and MOLT4 cell lysates.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3913 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRG1 antibody [EPR3913] (ab92496)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: BRG1 knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: Molt-4 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab92496 observed at 185 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab92496 was shown to recognize BRG1 when BRG1 knockout samples were used, along with additional cross-reactive bands. Wild-type and BRG1 knockout samples were subjected to SDS-PAGE. ab92496 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-BRG1 antibody [EPR3913] (ab92496)](https://www.abcam.com/ps/products/92/ab92496/Images/ab92496-107549-anti-brg1-antibody-epr3913-western-blot.jpg)
Western blot - Anti-BRG1 antibody [EPR3913] (ab92496)All lanes : Anti-BRG1 antibody [EPR3913] (ab92496) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 185 kDa
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![Anti-BRG1 antibody [EPR3913] (ab92496)](https://www.abcam.com/ps/products/92/ab92496/Images/ab92496-3-benefits-of-recombinant-antibodies.png)
Anti-BRG1 antibody [EPR3913] (ab92496)
