HRP Anti-BRG1 antibody [EPNCIR111A] (ab196315)
![HRP Anti-BRG1 antibody [EPNCIR111A] (ab196315)](https://www.abcam.com/ps/products/196/ab196315/Images/ab196315-310937-anti-brg1-antibody-epncir111a-hrp-western-blot.jpg "HRP Anti-BRG1 antibody [EPNCIR111A] (ab196315)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPNCIR111A] to BRG1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-BRG1 antibody [EPNCIR111A]
See all BRG1 primary antibodies -
Description
HRP Rabbit monoclonal [EPNCIR111A] to BRG1 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562, HeLa, NIH3T3, and PC12 whole cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPNCIR111A -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-BRG1 antibody [EPNCIR111A] (ab196315)All lanes : HRP Anti-BRG1 antibody [EPNCIR111A] (ab196315) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : Smarca4 (BRG1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 185 kDa
Observed band size: 185 kDa
Exposure time: 8 minutesab196315 was shown to specifically react with BRG1 in wild-type HAP1 cells as signal was lost in Smarca4 (BRG1) knockout cells. Wild-type and Smarca4 (BRG1) knockout samples were subjected to SDS-PAGE. Ab196315 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-BRG1 antibody [EPNCIR111A] (ab196315)](https://www.abcam.com/ps/products/196/ab196315/Images/ab196315-238251-anti-brg1-antibody-epncir111a-hrp-western-blot.jpg)
Western blot - HRP Anti-BRG1 antibody [EPNCIR111A] (ab196315)All lanes : HRP Anti-BRG1 antibody [EPNCIR111A] (ab196315) at 1/5000 dilution
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 :HeLa whole cell lysate (ab150035)
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 185 kDa
Observed band size: 185 kDa
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196315 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-BRG1 antibody [EPNCIR111A] (ab196315)](https://www.abcam.com/ps/products/196/ab196315/Images/ab196315-3-benefits-of-recombinant-antibodies.png)
HRP Anti-BRG1 antibody [EPNCIR111A] (ab196315)
