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Neuroscience Cell Type Marker Neural Stem Cell marker

Anti-PAX6 antibody (ab154253)

Anti-PAX6 antibody (ab154253)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PAX6
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PAX6 antibody
    See all PAX6 primary antibodies
  • Description

    Rabbit polyclonal to PAX6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-251 of Human PAX6 (UniProt ID: P26367).

  • Positive control

    • A431 and HepG2 whole cell lysate (ab7900); A549 cells and 293T cells
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • PAX
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Pancreas development

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab154253)
    Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab154253)
    Immunofluorescent analysis of paraformaldehyde-fixed A549 cells labeling PAX6 with ab154253 at 1/200 dilution. The image in the lower panel was merged with DNA probe.
  • Western blot - Anti-PAX6 antibody (ab154253)
    Western blot - Anti-PAX6 antibody (ab154253)
    All lanes : Anti-PAX6 antibody (ab154253) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa

  • Immunoprecipitation - Anti-PAX6 antibody (ab154253)
    Immunoprecipitation - Anti-PAX6 antibody (ab154253)
    Immunoprecipitation of PAX6 using 2 &microg of ab154253, and detected using ab154253 at 1/1000 dilution.
    Lane 1: 1000 &microg 293T whole cell lysate.
    Lane 2: 50 &microg 293T whole cell lysate
    Lane 3: 2 &microg preimmune control Rabbit IgG

    10% SDS-PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab154253)
    Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab154253)
    Immunofluorescent analysis of paraformaldehyde-fixed 293T cells labeling PAX6 with ab154253 at 1/250 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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