Anti-PAX6 antibody (ab154253)
Key features and details
- Rabbit polyclonal to PAX6
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAX6 antibody
See all PAX6 primary antibodies -
Description
Rabbit polyclonal to PAX6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-251 of Human PAX6 (UniProt ID: P26367).
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Positive control
- A431 and HepG2 whole cell lysate (ab7900); A549 cells and 293T cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunofluorescent analysis of paraformaldehyde-fixed A549 cells labeling PAX6 with ab154253 at 1/200 dilution. The image in the lower panel was merged with DNA probe.
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All lanes : Anti-PAX6 antibody (ab154253) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
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Immunoprecipitation of PAX6 using 2 µg of ab154253, and detected using ab154253 at 1/1000 dilution.
Lane 1: 1000 µg 293T whole cell lysate.
Lane 2: 50 µg 293T whole cell lysate
Lane 3: 2 µg preimmune control Rabbit IgG
10% SDS-PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed 293T cells labeling PAX6 with ab154253 at 1/250 dilution.