Anti-BRG1 antibody [EPNCIR111B] (ab133257)
![Anti-BRG1 antibody [EPNCIR111B] (ab133257)](https://www.abcam.com/ps/products/133/ab133257/Images/ab133257-269753-anti-brg1-antibody-epncir111b-western-blot.jpg "Anti-BRG1 antibody [EPNCIR111B] (ab133257)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR111B] to BRG1
- Suitable for: WB
- Knockout validated
- Reacts with: Rat, Human
Overview
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Product name
Anti-BRG1 antibody [EPNCIR111B]
See all BRG1 primary antibodies -
Description
Rabbit monoclonal [EPNCIR111B] to BRG1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Mouse BRG1.
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Positive control
- K562 cell lysate, HeLa cell lysate, Molt 4 cell lysate, PC12 cell lysate.
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General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPNCIR111B -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRG1 antibody [EPNCIR111B] (ab133257)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: BRG1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab133257 observed at 240 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab133257 was shown to recognize BRG1 when BRG1 knockout samples were used, along with additional cross-reactive bands. Wild-type and BRG1 knockout samples were subjected to SDS-PAGE. ab133257 and ab18058 (loading control to vinculin) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-BRG1 antibody [EPNCIR111B] (ab133257)](https://www.abcam.com/ps/products/133/ab133257/Images/ab133257-146678-anti-brg1-antibody-epncir111b-western-blot.jpg)
Western blot - Anti-BRG1 antibody [EPNCIR111B] (ab133257)All lanes : Anti-BRG1 antibody [EPNCIR111B] (ab133257) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Molt 4 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody. at 1/2000 dilution
Predicted band size: 185 kDa
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![Anti-BRG1 antibody [EPNCIR111B] (ab133257)](https://www.abcam.com/ps/products/133/ab133257/Images/ab133257-3-benefits-of-recombinant-antibodies.png)
Anti-BRG1 antibody [EPNCIR111B] (ab133257)
