Anti-Integrin beta 1 antibody [M032] - Extracellular domain (ab253282)
![Anti-Integrin beta 1 antibody [M032] - Extracellular domain (ab253282)](https://www.abcam.com/ps/products/253/ab253282/Images/ab253282-343946-antiingrinbeta1antibody-M032westernblothuman.jpg "Anti-Integrin beta 1 antibody [M032] - Extracellular domain (ab253282)")
Key features and details
- Mouse monoclonal [M032] to Integrin beta 1 - Extracellular domain
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Integrin beta 1 antibody [M032] - Extracellular domain
See all Integrin beta 1 primary antibodies -
Description
Mouse monoclonal [M032] to Integrin beta 1 - Extracellular domain -
Host species
Mouse -
Specificity
ab253282 detects a 120-150 kDa band corresponding to Integrin beta 1 on SDS-PAGE immunoblots of "Native" human A549, MDA-MB-231, and LNCaP cell lysates, as well as human breast, skin, and lung tissues.
The antibody weakly detects the denatured form of Integrin beta 1.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type within Human Integrin beta 1. The exact sequence is proprietary. (Proprietary antigen related to the extracellular region of human integrin beta 1 expressed in A431 epidermoid carcinoma cell line).
Database link: P05556 -
Positive control
- WB: A549, LNCaP and MDA-MB-231 cell lysates; human breast, lung and skin tissue lysates (Native). ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
M032 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Integrin beta 1 antibody [M032] - Extracellular domain (ab253282)All lanes : Anti-Integrin beta 1 antibody [M032] - Extracellular domain (ab253282) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) cell lysate
Lane 2 : LNCaP (human prostate cancer cell line) cell lysate
Lane 3 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate
Lane 4 : Human breast tissue lysate
Lane 5 : Human lung tissue lysate
Lane 6 : Human skin tissue lysate
Predicted band size: 88 kDaNative samples.
Primary for 1 hour at room temperature in PBS/5% non-fat milk/0.045 Tween 20.
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![Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody [M032] (ab253282)](https://www.abcam.com/ps/products/253/ab253282/Images/ab253282-343947-antiingrinbeta1antibodym032-immunocytochemistryimmunohistochemistry-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody [M032] (ab253282)Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for Integrin beta 1 (red) using ab253282 at 1/200 dilution in ICC/IF. The antibody was detected using goat anti-mouse Ig DyLight® 594.