Anti-SOX2 antibody (ab245291)
Key features and details
- Rabbit polyclonal to SOX2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SOX2 antibody
See all SOX2 primary antibodies -
Description
Rabbit polyclonal to SOX2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human SOX2 aa 150-200. The exact sequence is proprietary. NP_003097.1
Database link: P48431 -
Positive control
- WB: H520 and HEK-293T whole cell lysate. IP: SOX2 IP in H520 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245291 was affinity purified using an epitope specific to SOX2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SOX2 antibody (ab245291)All lanes : Anti-SOX2 antibody (ab245291) at 0.04 µg/ml
Lane 1 : H520 whole cell lysate at 50 µg
Lane 2 : H520 whole cell lysate at 15 µg
Lane 3 : H520 whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 minutes
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Immunoprecipitation - Anti-SOX2 antibody (ab245291)SOX2 was immunoprecipitated from H520 whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245291 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab245291 at 1 μg/ml.
Lane 1: ab245291 IP in H520 whole cell lysate.
Lane 2: Control IgG in H520 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
