Anti-SOX4 antibody (ab86809)
Key features and details
- Rabbit polyclonal to SOX4
- Suitable for: IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-SOX4 antibody
See all SOX4 primary antibodies -
Description
Rabbit polyclonal to SOX4 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
-
Immunogen
Synthetic peptide corresponding to Human SOX4 aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab103271) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab86809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IP Use a concentration of 5 µg/ml. IHC-P Use at an assay dependent concentration. Target
-
Function
Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif. -
Tissue specificity
Testis, brain, and heart. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 768313 Cow
- Entrez Gene: 6659 Human
- Entrez Gene: 20677 Mouse
- Omim: 184430 Human
- SwissProt: Q0VCF8 Cow
- SwissProt: Q06945 Human
- SwissProt: Q06831 Mouse
- Unigene: 643910 Human
see all -
Alternative names
- Ecotropic viral integration site 16 antibody
- EVI 16 antibody
- EVI16 antibody
see all
Images
-
Immunoprecipitation - Anti-SOX4 antibody (ab86809)
SOX4 was immunoprecipitated using 0.5mg Mouse Testis tissue, 5µg of Rabbit polyclonal to SOX4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Testis tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab86809.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 52kDa; SOX4
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX4 antibody (ab86809)IHC image of SOX4 staining in Human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86809, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX4 antibody (ab86809)This image is courtesy of an Abreview submitted by Carl Hobbsab86809 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab86809 at 1/400 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody. Sox4 can be observed in the nucleous of a sub-population of cells.
Protocols
Datasheets and documents
References (6)
ab86809 has been referenced in 6 publications.
- Du Q et al. Propofol inhibits proliferation, migration, and invasion but promotes apoptosis by regulation of Sox4 in endometrial cancer cells. Braz J Med Biol Res 51:e6803 (2018). PubMed: 29490000
- Liu Z et al. TGF-ß1 secreted by M2 phenotype macrophages enhances the stemness and migration of glioma cells via the SMAD2/3 signalling pathway. Int J Mol Med 42:3395-3403 (2018). PubMed: 30320350
- Jiao Y et al. SRY-Box Containing Gene 4 Promotes Liver Steatosis by Upregulation of SREBP-1c. Diabetes 67:2227-2238 (2018). PubMed: 30181160
- Bai JW et al. MicroRNA-320 inhibits cell proliferation and invasion in breast cancer cells by targeting SOX4. Oncol Lett 14:7145-7152 (2017). WB ; Human . PubMed: 29344145
- Zheng X et al. Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer. Nature 527:525-30 (2015). IHC-P ; Mouse . PubMed: 26560028
- Shim S et al. Cis-regulatory control of corticospinal system development and evolution. Nature 486:74-9 (2012). PubMed: 22678282
Images
-
Immunoprecipitation - Anti-SOX4 antibody (ab86809)
SOX4 was immunoprecipitated using 0.5mg Mouse Testis tissue, 5µg of Rabbit polyclonal to SOX4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Testis tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab86809.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 52kDa; SOX4
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX4 antibody (ab86809)IHC image of SOX4 staining in Human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86809, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX4 antibody (ab86809) This image is courtesy of an Abreview submitted by Carl Hobbs
ab86809 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab86809 at 1/400 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody. Sox4 can be observed in the nucleous of a sub-population of cells.
