Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse breast tissue sections labeling SOX10 with purified ab180862 at 1/500 (0.22 µg/ml). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180862).

Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab180862 at 1/50 (2.2 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab180862).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab180862 at 1/500 (0.22 µg/ml). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180862).

Immunohistochemistry (Frozen) analysis of rat cerebellum tissue sections labeling SOX10 with purified ab180862 at 1/50 (2.2 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab180862).

Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling SOX10 with ab180862 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180862).

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

This IHC data was generated using the same anti-SOX10 antibody clone, EPR4007-104, in a different buffer formulation (cat# ab180862).

Immunohistochemical analysis of paraffin-embedded Human fetal brain tissue labeling SOX10 with ab180862 at 1/100 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

This IHC data was generated using the same anti-SOX10 antibody clone, EPR4007-104, in a different buffer formulation (cat# ab180862).

Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling SOX10 with ab180862 at 1/100 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.