Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10429] to Arp3 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Arp3 antibody [EPR10429] - BSA and Azide free
    See all Arp3 primary antibodies

  • Description

    Rabbit monoclonal [EPR10429] to Arp3 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat, A549, Mouse spleen, Rat spleen, and Mouse brain lysates. IHC-P: human kidney, and mouse kidney tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    Ab240249 is the carrier-free version of ab181164. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240249 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Arp3 with purified ab181164 at 1:250 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181164)
  • Flow Cytometry - Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)
    Flow Cytometry - Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Arp3 with purified ab181164 at 1:30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181164)
  • Immunocytochemistry/ Immunofluorescence - Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)
    Immunocytochemistry/ Immunofluorescence - Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Arp3 with purified ab181164 at 1:50 dilution (5.06 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181164)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Arp3 with purified ab181164 at 1:250 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181164)
  • Immunocytochemistry/ Immunofluorescence - Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)
    Immunocytochemistry/ Immunofluorescence - Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)

    Immunofluorescence analysis of -20°C Acetone fixed HeLa cells labeling Arp3 with unpurified ab181164 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181164).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)

    Immunohistochemical analysis of paraffin embedded human kidney tissue labeling Arp3 with unpurified ab181164 at a 1/250 dilution. Prediluted HRP Rabbit IgG secondary was used. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181164).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)
    Anti-Arp3 antibody [EPR10429] - BSA and Azide free (ab240249)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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