Anti-TIM 3 antibody (ab185703)
Key features and details
- Rabbit polyclonal to TIM 3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TIM 3 antibody
See all TIM 3 primary antibodies -
Description
Rabbit polyclonal to TIM 3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human TIM 3 aa 1-250. Extracellular domain.
Database link: Q8TDQ0 -
Positive control
- WB: HepG2, BxPC-3, BT-474, U937, A-549, THP-1 cell lysates. Mouse spleen, lung and thymus. Rat spleen. IHC-P: Human lung cancer tissue, human tonsil tissue and mouse spleen tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody (ab185703)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue staining TIM 3 with ab185703 at 1/200 dilution.
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Western blot - Anti-TIM 3 antibody (ab185703)All lanes : Anti-TIM 3 antibody (ab185703) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : BxPC-3 cell lysate
Lane 3 : BT-474 cell lysate
Lane 4 : U-937 cell lysate
Lane 5 : Mouse lung tissue lysate
Lane 6 : Mouse thymus tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 10 seconds
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Western blot - Anti-TIM 3 antibody (ab185703)All lanes : Anti-TIM 3 antibody (ab185703) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A-549 cell lysate
Lane 3 : THP-1 cell lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Mouse lung tissue lysate
Lane 6 : Mouse thymus tissue lysate
Lane 7 : Rat spleen tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody (ab185703)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue staining TIM 3 with ab185703 at 1/200 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody (ab185703)Immunohistochemical analysis of paraffin-embedded human tonsil tissue staining TIM 3 with ab185703 at 1/200 dilution.
