Anti-TIM 3 antibody (ab47997)
Key features and details
- Goat polyclonal to TIM 3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TIM 3 antibody
See all TIM 3 primary antibodies -
Description
Goat polyclonal to TIM 3 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide:
C-KWYSHSKEKIQN
, corresponding to internal sequence amino acids 225-236 of Human TIM 3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TIM 3 antibody (ab47997)All lanes : Anti-TIM 3 antibody (ab47997) at 2 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 33 kDa
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Western blot - Anti-TIM 3 antibody (ab47997)Anti-TIM 3 antibody (ab47997) at 1 µg/ml + Human tonsil at 35 µg
Predicted band size: 33 kDa
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Western blot - Anti-TIM 3 antibody (ab47997)Anti-TIM 3 antibody (ab47997) at 0.3 µg/ml + human tonsil lysate at 35 µg
Predicted band size: 33 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
