Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 10, 2021

Anti-TIM 3 antibody [EPR20767] (ab210543)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20767] to TIM 3
Suitable for: IP, Flow Cyt, WB
Reacts with: Human

Product name

Anti-TIM 3 antibody [EPR20767]
See all TIM 3 primary antibodies
Description

Rabbit monoclonal [EPR20767] to TIM 3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Fc-tagged human TIM 3 recombinant protein (aa22-200); Daudi whole cell lysate. Flow Cyt: Human peripheral blood mononuclear cells (PBMCs). IP: Daudi whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20767
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-TIM 3 antibody [EPR20767] (ab210543)

Flow cytometric analysis of human peripheral blood mononuclear cells (PBMCs) labeling TIM 3 with ab210543 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Co-staining with anti-CD14 (y-axis). CD14+ PBMCs were positive for TIM 3 (PMID 22426209). Gated on total viable cells.
Western blot - Anti-TIM 3 antibody [EPR20767] (ab210543)

Anti-TIM 3 antibody [EPR20767] (ab210543) at 1/1000 dilution + Fc-tagged human TIM 3 recombinant protein (aa22-200) at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 33 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-TIM 3 antibody [EPR20767] (ab210543)

Anti-TIM 3 antibody [EPR20767] (ab210543) at 1/1000 dilution + Daudi (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 33 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Strong signal detected in Daudi cells after 15s exposure. Please note that under our experimental conditions (overexposure >3min), a faint band at ~70kDa was detected in Jurkat T cells (previously reported as a TIM 3 negative cell line PMID: 21807895).
Immunoprecipitation - Anti-TIM 3 antibody [EPR20767] (ab210543)

TIM 3 was immunoprecipitated from 0.35 mg of Daudi (human Burkitt's lymphoma cell line) whole cell lysate with ab210543 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab210543 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Daudi whole cell lysate 10 µg (Input).
Lane 2: ab210543 IP in Daudi whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab210543 in Daudi whole cell lysate.

Exposure time: 10 seconds.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-TIM 3 antibody [EPR20767] (ab210543)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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