Anti-TIM 3 antibody (ab233228)
Key features and details
- Rabbit polyclonal to TIM 3
- Suitable for: WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-TIM 3 antibody
See all TIM 3 primary antibodies -
Description
Rabbit polyclonal to TIM 3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-tag) corresponding to Human TIM 3 aa 1-250. (Expressed in E.coli).
Database link: Q8TDQ0 -
Positive control
- WB: Recombinant human TIM 3 protein; Human lung lysate; Pig lymph node tissue lysate; HepG2 and HEK-293T cell lysates. ICC/IF: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab233228 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TIM 3 antibody (ab233228)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for TIM 3 (green) using ab233228 at 20 μg/ml in ICC/IF, followed by FITC staining.
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Western blot - Anti-TIM 3 antibody (ab233228)Anti-TIM 3 antibody (ab233228) at 2 µg/ml + Recombinant human TIM 3 protein
Predicted band size: 33 kDa
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Western blot - Anti-TIM 3 antibody (ab233228)All lanes : Anti-TIM 3 antibody (ab233228) at 2 µg/ml
Lane 1 : Human lung lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 4 : Pig lymph node lysate
Predicted band size: 33 kDa
