Mouse TLR3 ELISA Kit (ab279413)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.26 ng/ml
- Range: 1.25 ng/ml - 80 ng/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TLR3 ELISA Kit
See all TLR3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 3% Inter-assay Sample n Mean SD CV% Extract 3 3.6% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
0.26 ng/ml -
Range
1.25 ng/ml - 80 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 84 84% - 85% Cell Lysate 83 82% - 84% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse TLR3 ELISA kit (ab279413) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse TLR3 protein in mouse cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TLR3 with 0.26 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Toll like Receptor 3 (TLR3) is a single-pass type I membrane protein. TLR3 is activated by double-stranded RNA which is a sign of viral infection. TLR3 acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response. Antibodies in ab279413 are raised to recognize a fragment of the luminal domain of TLR3.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse TLR3 Capture Antibody 1 x 600µl 10X Mouse TLR3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent BAR 1 x 6ml Mouse TLR3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent LS 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. -
Involvement in disease
Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 TIR domain. -
Domain
ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18. -
Post-translational
modificationsHeavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. -
Cellular localization
Endoplasmic reticulum membrane. Endosome membrane. - Information by UniProt
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Alternative names
- CD283
- CD283 antigen
- IIAE2
see all -
Database links
- Entrez Gene: 142980 Mouse
- SwissProt: Q99MB1 Mouse
- Unigene: 33874 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody benefitsTo learn more about the advantages of recombinant antibodies see here.
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Example of mouse TLR3 standard curve in 1X Cell Extraction Buffer PTR.The TLR3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TLR3 in mouse J774A.1 stimulated cell extract sample based on a 1,000 µg/mL extract load.The concentrations of TLR3 were measured in duplicate and interpolated from the TLR3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TLR3 concentration was determined to be 3.73 ng/mL in J774A.1 stimulated.
