Human Osteoactivin ELISA Kit (ab193711)
Key features and details
- Sensitivity: 45 pg/ml
- Range: 45 pg/ml - 12000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Osteoactivin ELISA Kit
See all GPNMB kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
45 pg/ml -
Range
45 pg/ml - 12000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 117.8 111% - 126% Plasma 116.5 107% - 126% Cell culture media 112.9 98% - 149% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Osteoactivin Human ELISA Kit (ab193711) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Osteoactivin in sera, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Osteoactivin coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Osteoactivin present in the samples. The wells are washed and biotinylated anti-Human Osteoactivin antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Osteoactivin bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated Human Osteoactivin detection antibody (lyophilized) 2 vials Human Osteoactivin Standard (lyophilized) 2 vials Pre-coated Human Osteoactivin Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Could be a melanogenic enzyme. -
Tissue specificity
Not restricted to the melanocytic lineage. -
Sequence similarities
Belongs to the PMEL/NMB family.
Contains 1 PKD domain. -
Developmental stage
Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines. -
Cellular localization
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Alternative names
- Glycoprotein (transmembrane) nmb
- Glycoprotein nmb
- Glycoprotein nmb like protein
see all -
Database links
- Entrez Gene: 10457 Human
- Omim: 604368 Human
- SwissProt: Q14956 Human
- Unigene: 190495 Human
Images
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Typical standard curve in Assay Diluent A
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Typical standard curve in Assay Diluent BData provided for demonstration purposes only. A new standard curve must be generated for each assay performed.