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Anti-Axl antibody [7E10] (ab130218)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [7E10] to Axl
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Axl antibody [7E10]
    See all Axl primary antibodies

  • Description

    Mouse monoclonal [7E10] to Axl

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human Axl. The exact sequence is proprietary.
    Sequence:

    Purified recombinant extracellular fragment of Human Axl fused with hIgGFc tag expressed in HEK293 cell line.


    Database link: P30530
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Flow Cyt: HeLa cells. ICC/IF: HeLa cells. WB: DU145, NCI-H1299 and HeLa whole cell lysates.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Axl antibody [7E10] (ab130218)
    Western blot - Anti-Axl antibody [7E10] (ab130218)
    All lanes : Anti-Axl antibody [7E10] (ab130218) at 1.947 µg/ml

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 2 : DU145 (human prostate carcinoma epithelial cell), whole cell lysate
    Lane 3 : NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 98 kDa
    Observed band size: 140,80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    The molecular weight observed was consistant with the literature (PMID: 19541935).

    Negative control: Jurkat (PMID: 28423548).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Axl antibody [7E10] (ab130218)
    Immunocytochemistry/ Immunofluorescence - Anti-Axl antibody [7E10] (ab130218)

    Immunofluorescent analysis of 4% methanol-fixed, 0.1% Triton X-100 permeabilized NCI-H1299 cells labeling Axl with ab130218 at 7.788 µg/mL, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on NCI-H1299 cell line.

    Negative control: Jurkat (PMID:28423548).

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab130218 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
    -ve control 2: ab179513 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

  • Flow Cytometry - Anti-Axl antibody [7E10] (ab130218)
    Flow Cytometry - Anti-Axl antibody [7E10] (ab130218)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte, Left panel) / HeLa (Human cervix adenocarcinoma epithelial cell, Right panel) labeling Axl using Anti-Axl antibody [7E10] (ab130218) at 0.974 µg/mL. Mouse monoclonal IgG isotype control (Black). The unlabeled control is cells without incubation with primary antibody or secondary antibody (Blue).

    Negative control: Jurkat.

    Gated on viable cells.

  • Anti-Axl antibody [7E10] (ab130218)
    Anti-Axl antibody [7E10] (ab130218)

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