Recombinant human IRAK-1 protein (Active) (ab271553)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human IRAK-1 protein (Active)
See all IRAK-1 proteins and peptides -
Biological activity
Specific activity ≥40 pmol/min/µg.
Assay was done in Kinase buffer containing 1 mM DTT using MBP substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min.
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Purity
>= 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
SVSLLQGARP FPFCWPLCEI SRGTHNFSEE LKIGEGGFGC VYRAVMRNTV YAVKRLKENA DLEWTAVKQS FLTEVEQLSR FRHPNIVDFA GYCAQNGFYC LVYGFLPNGS LEDRLHCQTQ ACPPLSWPQR LDILLGTARA IQFLHQDSPS LIHGDIKSSN VLLDERLTPK LGDFGLARFS RFAGSSPSQS SMVARTQTVR GTLAYLPEEY IKTGRLAVDT DTFSFGVVVL ETLAGQRAVK THGARTKYLK DLVEEEAEEA GVALRSTQST LQAGLAADAW AAPIAMQIYK KHLDPRPGPC PPELGLGLGQ LACCCLHRRA KRRPPMTQVY ERLEKLQAVV AGVPG -
Predicted molecular weight
40 kDa including tags -
Amino acids
186 to 530 -
Tags
His tag N-Terminus -
Additional sequence information
Genbank: NM_001569
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Preparation and Storage
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Alternative names
- AA48924
- Il1rak
- Interleukin 1 receptor associated kinase 1
see all -
Function
Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. -
Tissue specificity
Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. - Information by UniProt
Images
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Functional Studies - Recombinant Human IRAK-1 protein (Tagged) (ab271553)
Functional analysis of ab271553.
Specific activity ≥40 pmol/min/µg.
Assay was done in Kinase buffer containing 1 mM DTT using MBP substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min.
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SDS-PAGE - Recombinant Human IRAK-1 protein (Tagged) (ab271553)SDS-PAGE analysis of ab271553.