Anti-SOX2 antibody [EPR3130] - BSA and Azide free (ab248484)
![Anti-SOX2 antibody [EPR3130] - BSA and Azide free (ab248484)](https://www.abcam.com/ps/products/248/ab248484/Images/ab248484-4-anti-sox2-antibody-epr3130-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-SOX2 antibody [EPR3130] - BSA and Azide free (ab248484)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3130] to SOX2 - BSA and Azide free
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
Overview
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Product name
Anti-SOX2 antibody [EPR3130] - BSA and Azide free
See all SOX2 primary antibodies -
Description
Rabbit monoclonal [EPR3130] to SOX2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248484 is the carrier-free version of ab133337. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248484 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3130 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3130] - BSA and Azide free (ab248484)This data was developed using ab133337, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human fetal brain tissue labelling SOX2 with ab133337 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3130] - BSA and Azide free (ab248484)](https://www.abcam.com/ps/products/248/ab248484/Images/ab248484-3-anti-sox2-antibody-epr3130-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3130] - BSA and Azide free (ab248484)This data was developed using ab133337, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human glioma tissue labelling SOX2 with ab133337 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3130] - BSA and Azide free (ab248484)](https://www.abcam.com/ps/products/248/ab248484/Images/ab248484-2-anti-sox2-antibody-epr3130-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3130] - BSA and Azide free (ab248484)This data was developed using ab133337, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human brain tissue staining SOX2 with ab133337 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![Western blot - Anti-SOX2 antibody [EPR3130] - BSA and Azide free (ab248484)](https://www.abcam.com/ps/products/248/ab248484/Images/ab248484-399203-anti-sox2-antibody-epr3130-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-SOX2 antibody [EPR3130] - BSA and Azide free (ab248484)Anti-SOX2 antibody [EPR3130] (ab133337) at 1/500 dilution + NCCIT cell lysate at 10 µg
Secondary
Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?This data was developed using ab133337, the same antibody clone in a different buffer formulation.
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![Anti-SOX2 antibody [EPR3130] - BSA and Azide free (ab248484)](https://www.abcam.com/ps/products/248/ab248484/Images/ab248484-5-benefits-of-recombinant-antibodies.png)
Anti-SOX2 antibody [EPR3130] - BSA and Azide free (ab248484)
