Anti-SOX10 antibody [BLR080G] (ab265587)
![Anti-SOX10 antibody [BLR080G] (ab265587)](https://www.abcam.com/ps/products/265/ab265587/Images/ab265587-366218-AntiSOX10-antibody-immunohistochemistry-melanoma-human.jpg "Anti-SOX10 antibody [BLR080G] (ab265587)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR080G] to SOX10
- Suitable for: IHC-P, ICC, WB, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-SOX10 antibody [BLR080G]
See all SOX10 primary antibodies -
Description
Rabbit monoclonal [BLR080G] to SOX10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanIP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human SOX10 aa 416-466. The exact sequence is proprietary. NP_008872.1
Database link: P56693 -
Positive control
- IHC-P: Human melanoma tissue. Human breast carcinoma tissue. ICC: SK-MEL-28 cells. WB: MALME-3M, B16-F10, SK-MEL-28, MDA-MB-435 and UACC-62 whole cell lysate. IP: MALME-3M whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
BLR080G -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody (ab265587)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human melanoma tissue labeling SOX10 with ab265587 at 1/100 dilution. Detection: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody (ab265587)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling SOX10 with ab265587 at 1/100 dilution. Detection: DAB.
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Immunocytochemistry - Anti-SOX10 antibody (ab265587)Immunocytochemical analysis of formalin-fixed, paraffin-embedded SK-MEL-28 (Human melanoma cell line) cells labeling SOX10 with ab265587 at 1/100 dilution. Detection: DAB.
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Western blot - Anti-SOX10 antibody (ab265587)All lanes : Anti-SOX10 antibody [BLR080G] (ab265587) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : MALME-3M whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : SK-MEL-28 whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : MDA-MB-435 whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : UACC-62 whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 49 kDa
Exposure time: 10 secondsCells prepared using NETN lysis buffer.
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Western blot - Anti-SOX10 antibody (ab265587)All lanes : Anti-SOX10 antibody [BLR080G] (ab265587) at 1/1000 dilution
Lane 1 : TCMK whole cell lysate at 50 µg
Lane 2 : B16-F10 whole cell lysate at 15 µg
Lane 3 : NIH/3T3 whole cell lysate at 50 µg
Lane 4 : MALME-3M whole cell lysate at 15 µg
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 49 kDa
Exposure time: 3 secondsCells prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-SOX10 antibody (ab265587)SOX10 was immunoprecipitated from 1mg of MALME-3M whole cell lysate with ab265587 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab265587 at a 1/1000 dilution.
Lane 1: ab265587 IP in MALME-3M whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 secs.
Cells prepared using NETN lysis buffer.
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![Anti-SOX10 antibody [BLR080G] (ab265587)](https://www.abcam.com/ps/products/265/ab265587/Images/ab265587-7-benefits-of-recombinant-antibodies.png)
Anti-SOX10 antibody [BLR080G] (ab265587)
