Antibodies, Proteins, Kits and Reagents for Life Science

Anti-SOX10 antibody [BLR080G] (ab265587)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR080G] to SOX10
Suitable for: IHC-P, ICC, WB, IP
Reacts with: Mouse, Human

Product name

Anti-SOX10 antibody [BLR080G]
See all SOX10 primary antibodies
Description

Rabbit monoclonal [BLR080G] to SOX10
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human SOX10 aa 416-466. The exact sequence is proprietary. NP_008872.1
Database link: P56693
Positive control
- IHC-P: Human melanoma tissue. Human breast carcinoma tissue. ICC: SK-MEL-28 cells. WB: MALME-3M, B16-F10, SK-MEL-28, MDA-MB-435 and UACC-62 whole cell lysate. IP: MALME-3M whole cell lysate.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

BLR080G
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody (ab265587)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human melanoma tissue labeling SOX10 with ab265587 at 1/100 dilution. Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody (ab265587)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling SOX10 with ab265587 at 1/100 dilution. Detection: DAB.
Immunocytochemistry - Anti-SOX10 antibody (ab265587)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded SK-MEL-28 (Human melanoma cell line) cells labeling SOX10 with ab265587 at 1/100 dilution. Detection: DAB.
Western blot - Anti-SOX10 antibody (ab265587)

All lanes : Anti-SOX10 antibody [BLR080G] (ab265587) at 1/1000 dilution

Lane 1 : HEK-293T whole cell lysate
Lane 2 : MALME-3M whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : SK-MEL-28 whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : MDA-MB-435 whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : UACC-62 whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Predicted band size: 49 kDa

Exposure time: 10 seconds

Cells prepared using NETN lysis buffer.
Western blot - Anti-SOX10 antibody (ab265587)

All lanes : Anti-SOX10 antibody [BLR080G] (ab265587) at 1/1000 dilution

Lane 1 : TCMK whole cell lysate at 50 µg
Lane 2 : B16-F10 whole cell lysate at 15 µg
Lane 3 : NIH/3T3 whole cell lysate at 50 µg
Lane 4 : MALME-3M whole cell lysate at 15 µg

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Predicted band size: 49 kDa

Exposure time: 3 seconds

Cells prepared using NETN lysis buffer.
Immunoprecipitation - Anti-SOX10 antibody (ab265587)

SOX10 was immunoprecipitated from 1mg of MALME-3M whole cell lysate with ab265587 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab265587 at a 1/1000 dilution.
Lane 1: ab265587 IP in MALME-3M whole cell lysate.
Lane 2: Control IgG.

Exposure time: 3 secs.

Cells prepared using NETN lysis buffer.
Anti-SOX10 antibody [BLR080G] (ab265587)

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