Anti-SOX10 antibody [EPR4007] (ab155279)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4007] to SOX10
- Suitable for: ICC/IF, IHC-FoFr, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SOX10 antibody [EPR4007]
See all SOX10 primary antibodies -
Description
Rabbit monoclonal [EPR4007] to SOX10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF RatHumanIHC-FoFr MouseWB MouseRatHuman -
Immunogen
corresponding to Human SOX10 aa 400 to the C-terminus.
Database link: P56693 -
Positive control
- WB: Human brain, SH-SY5Y, A-375, C6, Mouse brain, Neuro-2a, and Rat brain lysates; ICC/IF: A-375 cells; Flow Cyt: A-375 cells. IHC-Fr: Mouse cerebellum
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4007 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab155279 at 1/50 (2.2 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.
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All lanes : Anti-SOX10 antibody [EPR4007] (ab155279) at 1/1000 dilution (Purified)
Lane 1 : Human brain lysates
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
Lane 5 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 49 kDa
Observed band size: 56-75 kDa why is the actual band size different from the predicted?The bands observed are consistent with what have been described in PMID: 21423190
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Immunocytochemistry/ Immunofluorescence analysis of C6 (Rat glial tumor glial cell) cells labeling SOX10 with purified ab155279 at 1:50 dilution (3.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow cytometry analysis of A-375 (human malignant melanoma cell line) cells labeling with purified ab155279 at 1/200 dilution ( 1ug/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) )(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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All lanes : Anti-SOX10 antibody [EPR4007] (ab155279) at 1/1000 dilution (unpurified)
Lane 1 : A-375 (human malignant melanoma cell line) lysate
Lane 2 : C6 (rat glial tumor cell line) lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDa
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Immunofluorescent staining of A-375 (human malignant melanoma cell line) cells labeling SOX10 with ab155279 (unpurified) at a 1/250 dilution.
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